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Laboratory Science

Researchers working in laboratories across the United States have made some of the most important medical breakthroughs of the last 250 years. These include the first use of general anesthesia and the discovery of the cause of yellow fever, as well as more recent innovations like recombinant DNA technology, GLP-1 inhibitors, and CAR-T Cell therapy. Learn about a handful of these important laboratory discoveries in this digital gallery.

1776-1826
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1776-1826

In the Early Republic, varying levels of medical training and limited professional standards yielded loosely organized practice of medicine. People sought out common therapeutic practices—including bloodletting, purging, surgery, and home remedies—from family members, communities, and physicians. Shaped by wars and limited public sanitation, smallpox, yellow fever, and other infectious diseases were major health concerns. The spread of smallpox inoculation, expanding medical education, and scientific inquiry advanced the medical profession. Explore a selection of NLM items produced between 1776 and 1826. Topics include wartime medicine, smallpox inoculation, Native American health and healing, yellow fever epidemics, botanicals and herbal remedies, and more.

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1827-1876
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1827-1876

American medicine became more organized during the 19th century as medical schools, hospitals, and professional societies expanded, though standards and training varied widely. Physicians practiced surgery, obstetrics, and emerging scientific medicine, while many people also turned to homeopathy and other alternative therapies. Infectious diseases such as cholera, smallpox, and rabies remained major threats, prompting growing interest in quarantine, sanitation, and public health. Significant advances included the use of ether anesthesia, improvements from Civil War medicine, and the development of professional nursing. Presented is an assortment of National Library of Medicine collection items printed between 1827 and 1876, covering topics such as homeopathy, nursing, infectious disease, administering ether, sanitation, women in medicine, Civil War medicine, and more.

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1877-1926
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1877-1926

Between 1877 and 1926, American medicine became increasingly scientific and professionalized as medical education and licensing became required by the states. Physicians relied on laboratory science, surgery, nursing care, and new diagnostic tools, while some Americans used widely advertised patent medicines alongside conventional treatments. Infectious diseases such as tuberculosis, influenza, rabies, and yellow fever remained major concerns, driving advances in public health and disease prevention, including the development of several vaccines. Germ theory, antiseptic surgery, X-rays, bacteriology, and the discovery of insulin transformed medical practice and outcomes. View this selection of items from the NLM collection produced from 1877 to 1926, which include works about pharmacology, surgery, infectious disease, public health, nursing, World War I medicine, and more.

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1927-1976
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1927-1976

By the mid-20th century, American medicine had become highly professionalized as physicians, nurses, hospitals, and public health agencies took on leading roles in healthcare. Medical practice increasingly relied on laboratory science, pharmaceuticals, vaccinations, and advanced technologies. These changes led to many Americans receiving care through hospitals, clinics, and expanding insurance systems. Infectious diseases declined as sulfa drugs, antibiotics, and vaccines transformed treatment and prevention. Chronic diseases, smoking-related illnesses, and environmental health concerns gained attention. Major medical breakthroughs included blood banking, open-heart surgery, organ transplantation, and DNA research. Explore a selection of NLM collection items produced between 1927 and 1976 about the discovery of DNA, substance use, World War II medicine, environmental health, computers in medicine, biotechnology, and more.

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1977-2026
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1977-2026

Since 1977, American medicine has become more technologically advanced and evidence based. Physicians, nurses, and allied professionals deliver specialized and team-based care. Patients access health services through hospitals, outpatient clinics, telemedicine, and digital health platforms, reflecting major changes in communications and access. Many health concerns from years past persist, like cancer and mental health disorders, while new ones have emerged, including the opioid epidemic, infectious diseases such as Ebola and COVID-19, and chronic conditions tied to lifestyle and aging populations. Advances in genomics, imaging, immunotherapy, and artificial intelligence continue to transform diagnosis and treatment, alongside major public health campaigns and public policy responses. Browse these NLM collection items created between 1977 and 2026, on subjects including HIV/AIDS, COVID-19, the opioid epidemic, mental health, telemedicine, public health communications, and more.

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Anatomy and Surgery
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Anatomy and Surgery

In the United States, the histories of anatomy and surgery have long been closely intertwined. Whereas early physicians often relied on limited anatomical knowledge and rudimentary surgical procedures to treat patients, later improvements in fields such as education, anesthesia, antibiotics, and blood transfusion made operations safer and more effective. Learn more about some of these important historical developments in this digital gallery.

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Cultures of Medicine
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Cultures of Medicine

Medicine has always involved cultural forces that impact the discipline as a whole. These forces impact the way in which individual physicians undertake their work and researchers discover various treatments for diseases. From stories about doctors and patients to the organizations that support their efforts, medical professionals are part of a broader system that helps to keep the entire nation healthy. Scroll through this digital gallery to learn more about the cultural history of medicine in the U.S.

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Diseases and Conditions
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Diseases and Conditions

Over the past 250 years, the list of diseases and conditions that affect people in the U.S. has changed dramatically. In the early Republic, cholera, yellow fever, and smallpox were among the leading causes of death. Today, advances in sanitation and public health mean that these illnesses have been eradicated or replaced, leaving chronic conditions such as cancer, diabetes, and heart disease as the most pressing health concerns. Learn about the changing history of diseases and related conditions in this digital gallery.

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Drugs and Treatments
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Drugs and Treatments

Drugs and therapeutic treatments have played an important role in U.S. medicine since the founding of the country in 1776. Whereas early physicians generally relied on traditional methods such as bloodletting and herbal remedies, later advances in vaccines, pharmaceuticals, antibiotics, and chemotherapy have helped to radically to improve patients’ health. Learn more about the development of some of these innovative drugs and treatments in this digital gallery.

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Laboratory Science
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Laboratory Science

Researchers working in laboratories across the United States have made some of the most important medical breakthroughs of the last 250 years. These include the first use of general anesthesia and the discovery of the cause of yellow fever, as well as more recent innovations like recombinant DNA technology, GLP-1 inhibitors, and CAR-T Cell therapy. Learn about a handful of these important laboratory discoveries in this digital gallery.

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Medical Technology
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Medical Technology

Since 1776, the history of medical technology in the United States has often reflected the broader scientific, industrial, and social progress of the nation. From the earliest surgical tools used during the Revolutionary War to more recent developments such as artificial heart valves and magnetic resonance imaging (MRI), U.S. technical innovation has always proceeded hand in hand with improvements in patient health. Learn more about some of these incredible inventions in this digital gallery.

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Military Medicine
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Military Medicine

Throughout its history, the United States has participated in numerous armed conflicts that have contributed to the growth of military medicine. From the War of Independence to the U.S. Civil War and more recent struggles in Europe, Vietnam, and Afghanistan, the country has relied on a dedicated group of doctors, nurses, and field medics to keep their troops healthy and combat ready. Learn more about their stories and the history of U.S. military medicine in this digital gallery.

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Mind and Brain
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Mind and Brain

Between the founding of the U.S. in 1776 and today, medical research into patients’ minds and brains has shifted from the field of philosophy to the natural and social sciences. During this time, researchers have become increasingly interested in the ways in which various physical and chemical structures in the brain contribute to human cognition. This in turn has revolutionized how we see learning, memory, and indeed mental health. Learn more about this historical transition in the digital gallery below.

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Preventive Medicine
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Preventive Medicine

Though we often think of preventive medicine as a recent historical development, the idea of taking proactive steps to ensure personal health is at least as old as the United States itself. For centuries, U.S. doctors and physicians have recommended a wide variety of herbal remedies, workout regimes, and dietary plans—each designed to help their patients live a healthier lifestyle. Learn about some of these innovative and unusual therapies in this digital gallery.

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Public Health
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Public Health

Public health has shaped the medical, political, and social life of the United States since the nation was first born two and a half centuries ago. Beginning with the efforts of local officials to contain an outbreak of Yellow Fever in Philadelphia in 1793, the U.S. government has consistently played an important role in improving living conditions and promoting healthy behaviors in communities all across the country. Learn more about the history of U.S. public health in this digital gallery.

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Audiovisuals
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Audiovisuals

Moving images serve as a vital tool for health education, document significant events and breakthroughs in medicine, and provide a way of sharing scientific scholarship. Explore this selection of audiovisual materials in the NLM collection, produced over the past century, originally in film, videocassette, and webcast.

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Born-Digital
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Born-Digital

Since the mid-20th century, digital media has transformed American health and medicine, changing how medical knowledge is created, shared, and preserved; empowering patients with greater access to information; enabling community action in the pursuit of wellbeing; and accelerating biomedical research and discovery. Learn about born-digital materials in the NLM collection, including websites and software, in this digital gallery.

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Manuscripts
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Manuscripts

Medical manuscripts document the development of American medicine. Physicians, researchers, students, hospitals, and public health officials manually record and collect observations, treatments, research findings, and institutional activities. These records support medical education and facilitate the exchange of knowledge. Today, medical manuscripts remain invaluable sources for understanding the scientific, social, and cultural history of health and medicine in the United States. Explore this selection of working notes and correspondence from clinicians, researchers, and patients.

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Still Images
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Still Images

From anatomical drawings and clinical photographs to public health posters and advertisements, still images help doctors diagnose illness, communicate scientific knowledge, educate the public, and shape perceptions of medicine. They have also served as records of medical practice, scientific discovery, and patient experience, preserving visual evidence of how health and medicine have evolved over time. Explore a selection of these still images from the collection in this digital gallery.

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Texts
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Texts

Over the past 250 years, medical texts have been the primary means of organizing and sharing medical knowledge gained in America. Textbooks, journals, and reference works have standardized medical education and clinical practice while supporting the professionalization of the field. They have enabled scientific communication, recorded shifting understandings of disease, and translated research into practice. Medical texts also provide a lasting scholarly record that continues to inform education, care, and historical research. Explore this assortment of medical texts from the NLM collection.

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34 Images

An illustration depicting three instruments for conducting experiments with heat, including a thermometer, a boiling box, and a set of water jugs.

Plain Discourses on the Laws or Properties of Matter, Thomas Ewell, New York, NY: Brisban & Brannan, 1806

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An illustration depicting three instruments for conducting experiments with heat, including a thermometer, a boiling box, and a set of water jugs.
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An illustration of a man standing on a short stool and holding a metal chain connected to a large device designed to generate electricity.

An Epitome of Electricity & Galvanism, Jacob Green, Philadelphia, PA: Jane Aitken, 1809

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An illustration of a man standing on a short stool and holding a metal chain connected to a large device designed to generate electricity.
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The title page of Statement of a Theory of Life, Founded on Observations & Experiments.

Statement of a Theory of Life, Founded on Observations & Experiments, David Porter, Brownsville, PA: W. Jackman, 1829

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The title page of Statement of a Theory of Life, Founded on Observations & Experiments.
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The title page of Experiments and Observations on the Gastric Juice, and the Physiology of Digestion.

Experiments and Observations on the Gastric Juice, and the Physiology of Digestion, William Beaumont, Plattsburg, NY: F. P. Allen, 1833

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The title page of Experiments and Observations on the Gastric Juice, and the Physiology of Digestion.
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An illustration of a parlor scene in which a man is staring intently at a woman seated in a chair while another man holds a sheet of paper in front of her face.

A New Theory of Animal Magnetism, Charles Ferson Durant, New York, NY: Wiley & Putnam, 1837

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An illustration of a parlor scene in which a man is staring intently at a woman seated in a chair while another man holds a sheet of paper in front of her face.
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The title page of Facts in Mesmerism, and Thoughts on its Causes and Uses.

Facts in Mesmerism, and Thoughts on its Causes and Uses, Charles Caldwell, Louisville, KY: Prentice and Weissinger, 1842

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The title page of Facts in Mesmerism, and Thoughts on its Causes and Uses.
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An illustration of several men in suits standing around a bed in which lies an unconscious patient with a bottle held up to his mouth

The First Public Demonstration of Surgical Anesthesia at Massachusetts General Hospital (Photomechanical Reproduction), Boston, MA, 1846

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An illustration of several men in suits standing around a bed in which lies an unconscious patient with a bottle held up to his mouth
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A page from a book that includes text and illustrations of the different developmental stages of tadpoles and frogs.

Twelve Lectures on Comparative Embryology, Louis Agassiz, Boston, MA: Redding & Co., 1849

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A page from a book that includes text and illustrations of the different developmental stages of tadpoles and frogs.
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A page from a book with two labeled illustrations of surgical instruments involving a chain loop which can be tightened.

Clinical Notes on Uterine Surgery, James Marion Sims, New York, NY: Wood, 1867

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A page from a book with two labeled illustrations of surgical instruments involving a chain loop which can be tightened.
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The weathered title page of The Application of Photography to Micrometry.

The Application of Photography to Micrometry, with Special Reference to the Micrometry of Blood in Criminal Cases, Joseph Woodward, Philadelphia, PA: J. B. Lippincott & Co., 1876

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The weathered title page of The Application of Photography to Micrometry.
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  • Exhibition - Visible Proofs: Forensic Views of the Body
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A black and white illustration of cholera bacteria growing on a gelatin plate.

Ten Days in the Laboratory with Dr. Koch, of Berlin, Geo W. Lewis, Buffalo, NY: Times Print, 1885

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A black and white illustration of cholera bacteria growing on a gelatin plate.
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Title page of a book with an Army Medical Library stamp.

New Manual of Alkaloidal Therapeutics, Adophe Burggraeve, Chicago, IL: Geo. T. Halla, 1888

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Title page of a book with an Army Medical Library stamp.
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An x-ray photograph showing the foot of a man wearing a boot

X-ray Photographs from The X-Ray: Or, Photography of the Invisible, William James Morton, New York, NY: American Technical Book Co., 1896

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An x-ray photograph showing the foot of a man wearing a boot
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  • Hidden Treasure: The National Library of Medicine
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A red book cover with black writing.

The Etiology of Yellow Fever, Walter Reed, Columbus, OH: Berlin Printing Company, 1901

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A red book cover with black writing.
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  • Exhibition - Politics of Yellow Fever in Alexander Hamilton's America
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The title page for Introduction to the Rarer Elements.

Introduction to the Rarer Elements, Phillip Browning, New York, NY: John Wiley & Sons, 1904

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The title page for Introduction to the Rarer Elements.
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A weathered book page with tape on one side; in the center of the text is the logo for the Carnegie Institute of Washington, which is a circle with an inset image of Andrew Carnegie and the words Carnegie Institute of Washington 1902 around the edge.

Sex-linked Inheritance in Drosophila, Thomas Hunt Morgan and Calvin Blackman Bridges, Washington, DC: Carnegie Institution of Washington, 1916

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A weathered book page with tape on one side; in the center of the text is the logo for the Carnegie Institute of Washington, which is a circle with an inset image of Andrew Carnegie and the words Carnegie Institute of Washington 1902 around the edge.
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Side-by-side pictures of a diabetic white boy, the left side shows him emaciated, the right side shows him healthier after treatment with insulin.

A Diabetic Child Treated with Insulin by Frederick Banting and Charles Best (Photoprint), J.P. Assal, World Health Organization, 1922

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Side-by-side pictures of a diabetic white boy, the left side shows him emaciated, the right side shows him healthier after treatment with insulin.
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  • MedlinePlus Health Topic: Diabetes
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A loose-leaf notebook page of handwritten notes.

Lab Notes from Michael Heidelberger and Oswald Avery's Experiments on Pneumococcus Bacteria and Polysaccharides, Michael Heidelberger and Oswald Avery, 1927

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A loose-leaf notebook page of handwritten notes.
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  • Profiles in Science - The Michael Heidelberger Papers
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A page of printed journal text.

"A Study of the Toxic Properties of Tuberculo-Proteins and Polysaccharides" from Journal of Experimental Medicine, Florence Sabin, New York, NY: Rockefeller Institute for Medical Research, 1931

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A page of printed journal text.
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  • Exhibition - Changing the Face of Medicine: Celebrating America's Women Physicians
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A movie still of a view through a camera lens of a white man in a white uniform preparing something in a laboratory.

Man Against Microbe, Iago Galdston and I.A. Jacoby (writers); F. Lyle Goldman (director), New York, NY: Metropolitan Life Insurance Company, 1932

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A movie still of a view through a camera lens of a white man in a white uniform preparing something in a laboratory.
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Published article titled The Structure of Proteins.

The Structure of Proteins, Linus Pauling, Washington, DC: Journal of the American Chemical Society, 1939

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Published article titled The Structure of Proteins.
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Black and white photograph of a woman looking through a microscope.

Rosalind Franklin with Microscope (Photoprint), 1955

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Black and white photograph of a woman looking through a microscope.
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Black and white film of cultured cells taken originally from a patient with cervical carcinoma.

The Hela Cell Strain: Cervical Carcinoma, Pomerat, Charles M., Washington, DC: Association of American Medical Colleges, 1956

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Black and white film of cultured cells taken originally from a patient with cervical carcinoma.
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A photograph of Barbara McClintock sitting at a lab bench, inspecting corn.

Barbara McClintock in Lab with Maize (Photoprint), Barbara McClintock, 1963

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A photograph of Barbara McClintock sitting at a lab bench, inspecting corn.
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A handwritten chart on taped together pieces of graph paper.

Genetic Code Chart, Marshall Nirenberg, 1965

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A handwritten chart on taped together pieces of graph paper.
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A printed page with an illustgation of an animo acid sequence below text.

Studies on the Principles That Govern the Folding of Protein Chains, Christian Anfinsen, Stockholm, Sweden: Nobel Foundation, 1973

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A printed page with an illustgation of an animo acid sequence below text.
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In a black and white photograph, a white woman and three white men talk around a coffeetable covered in papers that one of the white men is writing on; a chalkboard with writing appears in the background.

Maxine Singer, Norton Zinder, Sydney Brenner, and Paul Berg at the 1975 Asilomar Conference on Recombinant DNA (Photoprint), 1975

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In a black and white photograph, a white woman and three white men talk around a coffeetable covered in papers that one of the white men is writing on; a chalkboard with writing appears in the background.
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  • Profiles in Science - The Maxine Singer Papers
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A black and white, electron microscope image of oblong and circular bacteria.

Legionella Pneumophilis: The Bacteria that Causes Legionnaires' Disease (Photoprint), National Library of Medicine (NLM), Bethesda, MD, 1977

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A black and white, electron microscope image of oblong and circular bacteria.
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A printed page with a diagram showing five stages of ASV DNA synthesis.

Synthesis of ASV DNA, Harold Varmus, San Francisco, CA: University of California, San Franscisco, 1978

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A printed page with a diagram showing five stages of ASV DNA synthesis.
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A printed journal page with five in graphs.

"The Fat Cell Adenylate Cyclase System: Characterization and Manipulation of Its Biomodal Regulation by GTP" from Journal of Biological Chemistry, Werner Schlegel, Michael C. Lin, Dermot M.F. Cooper, Martin Rodbell , Rockville, MD: Journal of Biological Chemistry, 1979

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A printed journal page with five in graphs.
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  • Profiles in Science - The Martin Rodbell Papers
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First page of a report

Medication Errors in Labor and Delivery: Reducing Maternal and Fetal Harm, Institute for Safe Medication Practices, Harrisburg, PA: Pennsylvania Patient Safety Authority, 2009

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First page of a report
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A page from a report including a graph of the CDCs response to Ebola between March 2014 and July 2015 in Guinea, Liberia and Sierra Leone.

Outbreaks: Protecting Americans from Infectious Diseases, Jeffrey Levi, Laura M. Segal, Dara Alpert Lieberman, Kendra May, and Rebecca St. Laurent, Washington, DC: Robert Wood Johnson Foundation and Trust for America's Health, 2015

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A page from a report including a graph of the CDCs response to Ebola between March 2014 and July 2015 in Guinea, Liberia and Sierra Leone.
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Document cover page illustrated with DNA diagrams and a photograph of a doctor with a young child.

Drugs Against Cancer: Stories of Discovery and the Quest for a Cure, Kurt W. Kohn, Bethesda, MD: National Cancer Institute (NCI), 2022

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Document cover page illustrated with DNA diagrams and a photograph of a doctor with a young child.
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A screenshot of a website with text on the left and a photo of an African American woman wearing a t-shirt with the words All of Us Research Program on it

All of Us, NIH, 2026

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A screenshot of a website with text on the left and a photo of an African American woman wearing a t-shirt with the words All of Us Research Program on it
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  • NIH All of Us Website
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