The bilirubin blood test measures the level of bilirubin in the blood. Bilirubin is a yellowish pigment found in bile, a fluid made by the liver.
Bilirubin can also be measured with a urine test.
How the Test is Performed
A blood sample is needed.
How to Prepare for the Test
You should not eat or drink for at least 4 hours before the test. Your health care provider may instruct you to stop taking medicines that affect the test.
Many drugs may change the bilirubin level in your blood. Make sure your provider knows which medicines you are taking.
Why the Test is Performed
A small amount of older red blood cells are replaced by new blood cells every day. Bilirubin is left after these older blood cells are removed. The liver helps break down bilirubin so that it can be removed from the body in the stool.
A high level of bilirubin in the blood can lead to jaundice. Jaundice is a yellow color in the skin, mucus membranes, or eyes.
Jaundice is the most common reason to check bilirubin level. The test will likely be ordered when:
- The provider is concerned about a newborn's jaundice (most newborns have some jaundice)
- Jaundice develops in older infants, children, and adults
A bilirubin test is also ordered when the provider suspects a person has liver or gallbladder problems.
It is normal to have some bilirubin in the blood. A normal level is:
- Direct (also called conjugated) bilirubin: 0 to 0.3 mg/dL
- Total bilirubin: 0.3 to 1.9 mg/dL
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or may test different samples. Talk to your provider about the meaning of your specific test results.
What Abnormal Results Mean
In newborns, bilirubin level is higher for the first few days of life. Your child's provider must consider the following when deciding whether your baby's bilirubin level is too high:
- How fast the level has been rising
- Whether the baby was born early
- The baby's age
Jaundice can also occur when more red blood cells than normal are broken down. This can be caused by:
- A blood disorder called erythroblastosis fetalis
- A red blood cell disorder called hemolytic anemia
- Transfusion reaction in which red blood cells that were given in a transfusion are destroyed by the person's immune system
The following liver problems may also cause jaundice or a high bilirubin level:
- Scarring of the liver (cirrhosis)
- Swollen and inflamed liver (hepatitis)
- Other liver disease
- Disorder in which bilirubin is not processed normally by the liver (Gilbert disease)
The following problems with gallbladder or bile ducts may cause higher bilirubin levels:
Total bilirubin - blood; Unconjugated bilirubin - blood; Indirect bilirubin - blood; Conjugated bilirubin - blood; Direct bilirubin - blood
Ives NK, Mieli-Vergani G, Hadzic N, Newell S, Sugarman I. Gastroenterology. In: Rennie JM, ed. Rennie and Robsertson's Textbook of Neonatology. 5th ed. Philadelphia, PA: Elsevier; 2012:chap 29.
Pratt DS. Liver chemistry and function tests. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease: Pathophysiology/Diagnosis/Management. 10th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 73.
- Albumin - blood (serum) test
- Aplastic anemia
- Bile duct obstruction
- Biliary stricture
- Crigler-Najjar syndrome
- Dubin-Johnson syndrome
- Gilbert disease
- Glucose-6-phosphate dehydrogenase deficiency
- Hemolytic anemia
- Hemolytic disease of the newborn
- Hepatic encephalopathy
- Immune hemolytic anemia
- Liver disease
- Newborn jaundice
- Pernicious anemia
- Sickle cell anemia
- Transfusion reaction - hemolytic
- Wilson disease
Update Date 2/8/2015
Updated by: Laura J. Martin, MD, MPH, ABIM Board Certified in Internal Medicine and Hospice and Palliative Medicine, Atlanta, GA. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.