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Urine culture

A urine culture is a lab test to check for bacteria or other germs in a urine sample.

It can be used to check for a urinary tract infection in adults and children.

How the Test is Performed

Most of the time, the sample will be collected as a clean catch urine sample in your health care provider's office or your home. You will use a special kit to collect the urine.

A urine sample can also be taken by inserting a thin rubber tube (catheter) through the urethra into the bladder. This is done by someone in your provider's office or at the hospital. The urine drains into a sterile container, and the catheter is removed.

Rarely, your provider may collect a urine sample by inserting a needle through the skin of your lower abdomen into your bladder.

The urine is taken to a lab to determine which, if any, bacteria or yeast are present in the urine. This takes 24 to 48 hours.

How to Prepare for the Test

If possible, collect the sample when urine has been in your bladder for 2 to 3 hours.

How the Test Will Feel

When the catheter is inserted, you may feel pressure. A special gel is used to numb the urethra.

Why the Test is Performed

Your provider may order this test if you have symptoms of a urinary tract infection or bladder infection, such as pain or burning when urinating.

You also may have a urine culture after you have been treated for an infection. This is to make sure that all of the bacteria are gone.

Normal Results

"Normal growth" is a normal result. This means that there is no infection.

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.

What Abnormal Results Mean

A "positive" or abnormal test is when bacteria or yeast are found in the culture. This likely means that you have a urinary tract infection or bladder infection.

Other tests may help your provider know which bacteria or yeast are causing the infection and which antibiotics will best treat it.

Sometimes more than one type of bacteria, or only a small amount, may be found in the culture.


There is a very rare risk of a hole (perforation) in the urethra or bladder if your health care provider uses a catheter.


You may have a false-negative urine culture if you have been taking antibiotics.

Alternative Names

Culture and sensitivity - urine


Ban KM, Easter JS. Selected urologic problems. In: Marx JA, Hockberger RS, Walls RM, et al, eds. Rosen's Emergency Medicine: Concepts and Clinical Practice. 7th ed. Philadelphia, PA: Elsevier Mosby; 2009:chap 97.

Dean AJ, Lee DC. Bedside laboratory and microbiologic procedures. In: Roberts JR, Hedges JR, eds. Clinical Procedures in Emergency Medicine. 5th ed. Philadelphia, PA: Elsevier Saunders; 2009:chap 68.

Hooton TM, Bradley SF, Cardenas DD, et al. Diagnosis, prevention, and treatment of catheter-associated urinary tract infection in adults: 2009 International Clinical Practice Guidelines from the Infectious Diseases Society of America. Clin Infect Dis. 2010;50(5):625-663.

Update Date 11/1/2014

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