The quadruple screen test is a blood test done during pregnancy to determine whether the baby is at risk for certain birth defects.
This test is most often done between the 15th and 22nd weeks of the pregnancy. It is most accurate between the 16th and 18th weeks.
A blood sample is taken and sent to the lab for testing.
The test measures levels of four pregnancy hormones:
If the test does not measure levels of inhibin A, it is called the triple screen test.
To determine the chance of your baby having a birth defect, the test also factors in:
No special steps are needed to prepare for the test.
You may feel slight pain or a sting when the needle is inserted. You may also feel some throbbing at the site after the blood is drawn.
The test is done to find out if your baby might be at risk for certain birth defects, such as Down syndrome and birth defects of the spinal column and brain (called neural tube defects). This test is a screening test, so it does diagnose problems.
Certain women are at greater risk for having a baby with these defects, including:
Normal levels of AFP, hCG, uE3, and inhibin A.
Normal value ranges may vary slightly among different laboratories. Talk to your health care provider about the meaning of your specific test results.
An abnormal test result does NOT mean that your baby definitely has a birth defect. Often, the results can be abnormal if your baby is older or younger than your doctor or nurse had thought.
If you have an abnormal result, you will have another ultrasound to check the age of the developing baby.
More tests and counseling may be recommend if the ultrasound shows a problem. However, some people choose not to have any more tests done, for personal or religious reasons. Possible next steps include:
During pregnancy, increased levels of AFP may be due to a problem with the developing baby, including:
High AFP can also mean that you're carrying more than one baby.
Low levels of AFP and estriol and high levels of hCG and inhibin A may be due to a problem such as:
The quadruple screen can have false-negative and false-positive results (although it is slightly more accurate than the triple screen). More tests are needed to confirm an abnormal result.
If the test is abnormal, you may want to talk to a genetic counselor.
Quad screen; Multiple marker screening; AFP plus; Triple screen test; AFP maternal; MSAFP; 4-marker screen
Simpson JL, Richards DS, Otano L, et al. Prenatal genetic diagnosis In: Gabbe SG, Niebyl JR, Simpson JL, et al, eds. Obstetrics: Normal and Problem Pregnancies. 6th ed. Philadelphia, Pa: Saunders Elsevier; 2012:chap 11.
Williams DE, Pridjian G. Obstetrics. In: Rakel RE, ed. Textbook of Family Medicine. 8th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 21.
ACOG Committee on Practice Bulletins. ACOG practice bulletin no. 77: screening for fetal chromosomal abnormalities. Obstet Gynecol. 2007;109:217-227.
Anderson CL, Brown CE. Fetal chromosomal abnormalities: Antenatal screening and diagnosis. Am Fam Physician. 2009;79:117-123.
Driscoll DA, Morgan MA, Schulkin J. Screening for Down syndrome: changing practice of obstetricians. Am J Obstet Gynecol. 2009;200:459.e1-459.e9.
Updated by: Susan Storck, MD, FACOG, Chief, Eastside Department of Obstetrics and Gynecology, Group Health Cooperative of Puget Sound, Bellevue, Washington; Clinical Teaching Faculty, Department of Obstetrics and Gynecology, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.
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